RQMICRO INTRODUCES NEXT GENERATION MICROBIOLOGY DETECTION
April 18, 2017 - rqmicro AG, based in Zurich, Switzerland, develops and markets proprietary reagents and innovative instruments for rapid microbial tests in water and food. rqmicro uses the great potential of microfluidics to deliver a product, that allows target cells to be separated from a sample with high efficiency, using immunomagnetic separation (IMS).
The vision of rqmicro is that consumers worldwide benefit from water and food which is free from microbiological contamination. A crucial step towards realizing that vision is to detect various pathogens in a quick and accurate way. This is why rqmicro’s team developed a technology that delivers fully quantitative test results at an unmatched speed. rqmicro’s customers in the water industry will realize significant financial benefits using the rapid microbiological test platform and make the world a safer place.
The lab-on-a-chip technology from rqmicro AG is able to isolate Legionella from water samples in just one hour. The automated and highly specific method is based on immunomagnetic separation and microfluidics. Results correlate to the plating method with higher sensitivity and minimal standard deviation.
Innovative Instrument of Rapid Sample Preparation
CellStream instrumentation works in conjunction with reagent kits and microfluidic cartridges for a complete solution, enabling the user to isolate and stain target cells (e.g. Legionella) from a water sample. The concentrated and purified sample can be directly quantified with a standard flow cytometer.
Disposable and convenient-to-use microfluidic cartridges enable the user to perform fully automated immunomagnetic separations (IMS) at a high throughput, resulting in isolated samples with unmatched purity and very high recovery rates. Four samples can be processed in parallel with never-before-seen process stability.
Water laboratories benefits from integration of the CellStream in their workflow due:
- Reduce sample preparation time - skip cultivation and enrichment steps and prepare a clean sample of target bacteria in less than 1 hour.
- Minimal manual intervention reduces operator bias - our automated workflow generates consistent results.
- Keep maximum analytical flexibility - the IMS process leaves cells completely intact (no lysis) and therefore provides a great basis for any analytical method.
- One instrument processes up to 8 samples in 1 hour.
Legionella pneumophila SG1 CellStream Kit
Ultrafast Detection Including Viability Assessment
rqmicro developed this highly specific kit to simplify and expedite the detection of Legionella pneumophila SG1 in water samples. Together with CellStream it opens up a new chapter in the field of pathogen detection and water quality.
The advantages that rqmicro kit provides to its users are:
- Purification of Legionella in 1 hour
- High specificity and sensitivity
- Superior monoclonal antibody pools
- Easy handling
- Adaptable to any matrix
- Flexible integration into any workflow
- Compatible with flow cytometry, cultivation method, PCR or microscopy
Rapid sample preparation kit for flow cytometric sample analysis:
rqmicro’s kits are suitable for use with all standard flow cytometers. Using flow cytometer and CellStream, water labs generate new insights into water systems due to live-dead staining of specific pathogens and the possibility for real-time monitoring of processes, e.g. disinfection.
Next to flow cytometry, CellStream can support many other analytical methods (e.g. cultivation method, PCR, fluorescence microscopy) or help you purify or treat samples for further processing.
Equivalence of rqmicro’s method to ISO cultivation method:
- Immunomagnetic separation and flow cytometry achieve > 98% correlation to current gold standard (cultivation method) with a 5-10 fold higher sensitivity
- Equivalent/more accurate results achieved in less than 2 hours instead of 10 days
Detection of viable but not cultivable bacteria cells (VBNC)
rqmicro’s technology in combination with flow cytometer reveals that viable Legionella bacteria are still present after 60 minutes. At the same time, plating (cultivation method) does not detect VBNC bacteria (VBNC - viable but non-culturable). The presence of VBNC bacteria does therefore question the accuracy of cultivation method, in comparison with rqmicro’s method.
Rapid Legionella test application in hospital tap water
Here is one of the cases where rqmicro’s technology proved its excellence. The water system of a hospital was periodically flushed during 14 days with hot water in order to eliminate a high Legionella contamination. By using rqmicro’s rapid Legionella testing service, it was possible to monitor the progress of the disinfection measures. It was possible to adapt and optimize the process, until the result was satisfactory. By conventional cultivation method the first testing results would have been available after 14 days, at which point rqmicro’s customer had already peace of mind. An accurate and fast monitoring of hygienisation measures with rqmicro’s technology provides 90% downtime reduction, saves money and protects you from the risk of having the often undetected VBNC-state Legionella cells.
Current test kits in development:
The first commercial kit for L.p. SG 1 will complemented in the near future with kits for
- Legionella pneumophila SG 1-15
- Legionella spp.
- Pseudomonas aeruginosa
- Giardia spp. and Cryptosporidium spp.
- E. coli O157
rqmicro AG, based in Zurich, Switzerland, develops and markets proprietary reagents and innovative instruments for rapid microbial tests in water and food.
For further information about rqmicro, visit: www.rqmicro.com
Media contact information
Marketing & Sales Specialist
+41 79 557 66 77
rqmicro AG Brandstrasse 24 8952 Schlieren Switzerland
Tel: +41 44 512 51 51
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