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LABVOLUTION 2019, 21 - 23 May
Homepage>Conference program >Automated two-step purification of antibodies
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Automated two-step purification of antibodies

Location & Language

Language

English, German

Event Details

Type of event

Lecture

Topic

Applications/Research Biotechnology, Laboratory workflow optimization, Life Sciences

Event Host

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Description

Antibodies (immunoglobulins, Ig's) are part of the immune system. They can identify and bind particular antigens thereby neutralizing them. Due to their specific target recognition/binding function they have a significant importance in the biotechnology and pharmaceutical industry. Key applications are the diagnosis and treatment of diseases. Besides, antibodies are also the crucial components in numerous research applications such as Western Blots and immunoassays. Quality and purity of the IgG is crucial for these applications. The purification of antibodies involves two to three steps:
1. capture step
2. optional intermediated step
3. polishing step
The transition from one to another step generally involves manual interaction and thus is time consuming. Automation by combining affinity chromatography step with a gel filtration step to exchange the buffer of the purified antibodies increases the efficiency and optimize the workflow.

Speaker

  Stephanie Runde

Stephanie Runde

KNAUER Wissenschaftliche Geräte GmbH

Stephanie Runde, born in Munich (1984) studied Biochemistry at Technical University of Munich, where she got her PhD in 2014. And She works since then as product manager at KNAUER Wissenschaftliche Geräte ...

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