Cytation 5 with Augmented Microscopy combines automated digital widefield microscopy with conventional multi-mode microplate reading. This unique patented design provides rich phenotypic cellular information with well-based quantitative data. This single instrument platform can process workflows that would traditionally require multiple instruments and software interfaces, and is simple to setup and operate. With up to 60x magnification, the microscopy module provides high-quality cellular and sub-cellular imaging in fluorescence, brightfield, color brightfield and phase contrast. The multi-mode microplate reader features BioTek's patented Hybrid Technology, which incorporates variable bandwidth monochromator optics and high sensitivity filter-based detection optics for unmatched versatility and performance. Live cell imaging and multi-mode assays are optimized with incubation to 65 °C and shaking, plus available CO2/O2 control and dual reagent injectors. Gen5 3.0 software provides automated image capture and analysis, plus annotation and movie maker functions. Augmented Microscopy is the combination of all of these features in one compact system.