Increasing the stability of polypeptides with genetic engineering
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Several methods for increasing enzyme stability can be applied. The most common option isto use stabilizing additives in the enzyme storage buffer. But this alone may not be sufficientto increase the stability at temperatures above freezing. Another process used to improveproteins' long-term stability is drying via lyophilization. This method is complicated and canresult in loss of enzyme activity.We have used genetic engineering to increase protein stability at room temperature by addinga special tag (European Patent EP2501716, US Patent No 9,321,999) to several enzymes (e.g.DNA polymerases, reverse transcriptases, RNase inhibitors). These genetically modifiedproteins can be stored at up to +35C for at least 4 weeks without any loss of enzymaticactivity when compared to the same enzymes that were kept at -20C.
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