LABVOLUTION 2019, 21 - 23 May
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Optical Oxygen Measurements in Cell Tissue Using Phosphorescent Microspheres

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Hall 20, Stand D30



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Digitalization , Life Sciences, smartLAB / Lab of the future

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Molecular oxygen is a very important substance regarding the metabolism of cells. Compliance to certain oxygen concentrations is crucial for a successful cell breeding. Measuring of oxygen provides information about the vitalities of cell cultures. However, to receive meaningful results it is necessary to measure within the cell culture instead within the supernatant medium. Phosphorescent microspheres that are overgrown by cells are suitable probes for this purpose. A special phase modulation technique applied to the excitation light allows a separation of the oxygen-dependent probe signal from background fluorescence that unavoidably arises from biological samples. This technique enables reliable real time measurements even for faint and superposed probe signals. Application examples are liver cell toxicity tests and oxygen monitoring during the breeding of cartilage tissue in a 3D bioreactor.


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