LABVOLUTION 2019, 21 - 23 May

Cell Lysis

Cell Lysis (Mammalian, Yeast, E. coli)

Logo Cell Lysis

Product description

Cell Lysis
AFA Ultrasonic Technology is unique in its ability to control the amount of acoustic energy delivered to samples such as cells. By utilizing the control of the AFA to vary the energy that is delivered to a sample, it is possible to gently disrupt the cell membranes of mammalian cells (e.g., G-protein coupled receptor assays) or abruptly disrupt the cell walls of bacteria (e.g., total protein extraction).

Cell lysis differs greatly depending on the species the cells are derived from (e.g., plant cells are significantly harder to lyse than mammalian cells). Consequently, lysis buffer choice is a critical component in the cellular lysis process. Typically, a lysis buffer is chosen by acknowledging the stabilization characteristics of the target molecule, rather than for the lytic capabilities of the buffer or intended downstream analytical methods. While this improves the stability of the target molecules once extracted, it does not assure the most efficient extraction, or compatibility with analytical methods. The controlled mechanical energy provided by AFA improves the efficiency of cellular lysis independent of buffer constituents. Therefore, AFA provides the flexibility to homogenize cells and efficiently extract proteins, RNA, DNA, or other targets in buffers optimized for intended analytical methods.

Product website

Hall 19, Stand B76

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