21 - 23 May
Cell LysisAFA Ultrasonic Technology is unique in its ability to control the amount of acoustic energy delivered to samples such as cells. By utilizing the control of the AFA to vary the energy that is delivered to a sample, it is possible to gently disrupt the cell membranes of mammalian cells (e.g., G-protein coupled receptor assays) or abruptly disrupt the cell walls of bacteria (e.g., total protein extraction).Cell lysis differs greatly depending on the species the cells are derived from (e.g., plant cells are significantly harder to lyse than mammalian cells). Consequently, lysis buffer choice is a critical component in the cellular lysis process. Typically, a lysis buffer is chosen by acknowledging the stabilization characteristics of the target molecule, rather than for the lytic capabilities of the buffer or intended downstream analytical methods. While this improves the stability of the target molecules once extracted, it does not assure the most efficient extraction, or compatibility with analytical methods. The controlled mechanical energy provided by AFA improves the efficiency of cellular lysis independent of buffer constituents. Therefore, AFA provides the flexibility to homogenize cells and efficiently extract proteins, RNA, DNA, or other targets in buffers optimized for intended analytical methods.
truCOLLEC, a novel blood collection, stabilization, and DNA extraction system specifically designed for use in NGS
Genetic analysis from DBS cards has become routine in many laboratories, but the potential offered by NGS to deliver a wealth of addition clinically useful information ...
truXTRA cfDNA Kits
designed to standardize circulating cell-free DNA extraction;
an active AFA-based, high-throughput, high-yield system
Adaptive Focused Acoustics (AFA)
Accurate: Enables equivalent MALDI-TOF species identification when compared to Myco Ex
Simple: Easy to use - minimal training ...
Next-Gen Sequencing has quickly become one of the most important tools in genomics research and nucleic acid fragmentation is a crucial first step in the sequencing ...
Formalin Fixation and Paraffin Embedding (FFPE) of tissues is rapidly being adopted for routine molecular diagnostics. The extreme formaldehyde fixation and tissue ...
truChIP Chromatin Shearing Kits provide optimized, flexible, and universal protocols for chromatin shearing with high performance Focused-ultrasonicators, enabling ...