21 - 23 May
Next-Gen Sequencing has quickly become one of the most important tools in genomics research and nucleic acid fragmentation is a crucial first step in the sequencing workflow. While there are a variety of methods available to fragment nucleic acids, mechanical shearing remains the method of choice for achieving high sensitivity and unbiased results. Adaptive Focused Acoustics (AFA) technology is firmly established as the fragmentation method of choice for NGS, and Covaris continues to innovate the tools required to shear DNA and RNA without GC bias or thermal damage.The Covaris AFA process is conducted under isothermal conditions, ensuring the integrity of the nucleic acid sample is maintained and providing high recovery of double-stranded DNA. Combined with the specifically engineered AFA Tubes it is possible to precisely and accurately fragment DNA and RNA to the 100 - 1500bp range (microTUBE), or 2 - 5kb range (miniTUBE). Focused-ultrasonicators range from the inexpensive M-Series to the single-tube full-power S-Series to higher throughput E and L-Series instruments.For sequencing applications requiring longer DNA fragments, Covaris developed the patented g-TUBE. Generating shearing forces through centrifugation, the g-TUBE produces your selected fragment length in the the 6 kb - 20 kb range. Apart from a benchtop centrifuge, no other equipment in needed.Mechanical shearing with AFA technology is the gold standard for nucleic acid fragmentation prior to NGS library preparation. All major sequencing instrument providers recommend the use of Covaris technology to obtain the highest quality data:
truCOLLEC, a novel blood collection, stabilization, and DNA extraction system specifically designed for use in NGS
Genetic analysis from DBS cards has become routine in many laboratories, but the potential offered by NGS to deliver a wealth of addition clinically useful information ...
truXTRA cfDNA Kits
designed to standardize circulating cell-free DNA extraction;
an active AFA-based, high-throughput, high-yield system
AFA Ultrasonic Technology is unique in its ability to control the amount of acoustic energy delivered to samples such as cells. By utilizing the control ...
Adaptive Focused Acoustics (AFA)
Accurate: Enables equivalent MALDI-TOF species identification when compared to Myco Ex
Simple: Easy to use - minimal training ...
Formalin Fixation and Paraffin Embedding (FFPE) of tissues is rapidly being adopted for routine molecular diagnostics. The extreme formaldehyde fixation and tissue ...
truChIP Chromatin Shearing Kits provide optimized, flexible, and universal protocols for chromatin shearing with high performance Focused-ultrasonicators, enabling ...