LABVOLUTION Digital Edition | September 2021
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Droplet Digital PCR Technology

Logo Bio-Rad Laboratories


Bio-Rad Laboratories

Exhibitor details
Exhibitor details
Logo Droplet Digital PCR Technology

Product description

Digital PCR is a breakthrough technology that provides ultrasensitive and absolute nucleic acid quantification. It is particularly useful for low-abundance targets, targets in complex backgrounds, allelic variants (SNPs) and for monitoring subtle changes in target levels that cannot be detected with real-time PCR. This technology is now approved for use in in vitro diagnostics.

Benefits of Droplet Digital PCR

Absolute quantification — provides an absolute count of target DNA copies per input sample without the need for standard curves

Highest precision and sensitivity — enriched template concentration in target-positive droplets allows higher precision and sensitivity than real-time PCR

High throughput — simple and easy-to-use workflow with 96-sample throughput does not require multiple dilution steps

Removal of PCR efficiency bias — error rates are reduced by removing the reliance on amplification efficiency of qPCR

Applications of Droplet Digital PCR

Copy number variation (CNV) - accurate CNV quantification in single wells

Next generation sequencing - amplification and accurate quantification of NGS libraries with validation of sequencing results

Detection of rare sequences in cancer and disease - detection of mutations and viral load below levels detectable by current tests

Gene expression analysis - detection of rare mRNAs and miRNAs with rapid turnover

Pathogen detection and microbiome analysis - viral load and pathogen detection in organ transplants, soil, and food

Single cell analysis - high sensitivity make it a robust tool for single-cell analyses

Product website

Hall 19, Stand B78

Accelerating Scientific Discovery.
(Main stand)

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