LABVOLUTION Digital Edition |
Digital PCR is a breakthrough technology that provides ultrasensitive and absolute nucleic acid quantification. It is particularly useful for low-abundance targets, targets in complex backgrounds, allelic variants (SNPs) and for monitoring subtle changes in target levels that cannot be detected with real-time PCR. This technology is now approved for use in in vitro diagnostics.
Benefits of Droplet Digital PCR
Absolute quantification — provides an absolute count of target DNA copies per input sample without the need for standard curves
Highest precision and sensitivity — enriched template concentration in target-positive droplets allows higher precision and sensitivity than real-time PCR
High throughput — simple and easy-to-use workflow with 96-sample throughput does not require multiple dilution steps
Removal of PCR efficiency bias — error rates are reduced by removing the reliance on amplification efficiency of qPCR
Applications of Droplet Digital PCR
Copy number variation (CNV) - accurate CNV quantification in single wells
Next generation sequencing - amplification and accurate quantification of NGS libraries with validation of sequencing results
Detection of rare sequences in cancer and disease - detection of mutations and viral load below levels detectable by current tests
Gene expression analysis - detection of rare mRNAs and miRNAs with rapid turnover
Pathogen detection and microbiome analysis - viral load and pathogen detection in organ transplants, soil, and food
Single cell analysis - high sensitivity make it a robust tool for single-cell analyses
Accelerating Scientific Discovery.
The complete V3 Western Workflow incorporates traditional blotting techniques with innovative tools to quickly check electrophoresis results and blot transfer quality ...