The versatile FluidFM BOT system opens the door to single cell experiments that have not been possible with conventional methods. Built around the patented FluidFM hollow probe technology, the highly automated system allows cell manipulation on the single cell level, and this in a non-destructive and cell-context preserving manner.
Inject a vast variety of compounds such as DNA, RNA and plasmids directly into nuclei or cytoplasms of adherent cells. This is done in a ”gentle”, less stressful manner than with most other delivery methods, resulting in cell viability of around 95%. In addition, it is possible to measure the injected femtoliter volumes (fL) and inject 100 to 300 cells/hour depending on the cell types.
Extract the content of individual cells directly in their native environment while preserving the cellular context and without affecting cell viability. The procedure is non-destructive and therefore makes it possible to perform cell biopsies from the same single cell over different time points.
FluidFM cell isolation:
Isolate single adherent or suspended cells by placing the selected cell in a new recipient. Combined with FluidFM nano-injection, FluidFM cell isolation is a powerful tool for scientists interested in fast and efficient production of newly transfected monoclonal cell lines.
Print spots, high-density arrays or complex patterns with many types of biological particles with nanometer precision, useful for example in biosensing to create ad-hoc protein or DNA arrays.