LABVOLUTION 2019, 21 - 23 May
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PCRbeam Fast PCR Detection Kit

Logo PCRbeam Fast PCR Detection Kit

Product description

- Sensitive detection of PCR, LAMP, RPA gene-specific products
- No gel loading after PCR, no ethidium bromide handling
- Saved costs compared to qPCR-based detection methods
- Fast and easy procedure with little hands-on time


highQu PCRbeam Fast PCR Detection Kit is a convenient tool for fast detection of gene-specific amplification products obtained by PCR, LAMP or RPA. The detection is based on immunological reaction driven by Biotin and FITC (fluorescein isothiocyanate), thus the amplified DNA shall include Biotin and FITC labels.
The PCR amplification has to be performed with one primer labeled with FITC at 5'-end and one primer labeled with Biotin at 5'-end. Alternatively, the use of one of the labeled primers can be replaced by gene-specific FITC or Biotin-labeled probe. Kit includes PCRbeam Membrane Strips that are coated with biotin-ligand on the test band and an anti-rabbit antibody on the control band. The bottom part of the strip which is used for sample application contains an anti-FITC antibody attached to gold particles. PCRbeam Detection Buffer is Tris-buffered saline enabling the detection.
The PCRbeam Fast PCR Detection Kit can be applied for established tests or home-brew assays as a fast and sensitive yes/no detection method. The detection sensitivity is up to 100 fold higher than the one achievable with ethidium bromide stained gels what provides an environment friendly save and economical alternative to the use of mutagen stains.
For establishing sensitive PCR-based tests before PCRbeam detection we recommend the use of hot-start PCR enzymes or master mixes, like highQu ALLin Hot Start Taq Mastermix or ALLin Hot Start Taq DNA Polymerase.

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Hall 20, Stand A12, (4)

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