LABVOLUTION Digital Edition |
SERVA Lightning Red is a fluorescent dye for rapid labelling of proteins prior to SDS PAGE, making any staining and washing steps after electrophoresis unnecessary. In addition the dye is fully compatible with mass spectrometry and other downstream methods e. g. Western Blotting. SERVA Lightning Red is compatible with all additives typically used for sample solubilisation and protein extraction, including reductants like dithiothreitol (DTT) and dithioerythreitol (DTE).The shift of the electrophoretic mobility in SDS electrophoresis is very low; migration differences between labelled and non-labelled proteins could not be detected. This is due to the small mass addition of only 288 Da per bound molecule and the low hydrophobicity of the dye molecule. Traditionally silver staining of PAGE gels is preferred to other staining methods when highest sensitivity of detection is required. However silver stained bands exhibit very quickly saturation. Therefore quantification of proteins is not feasible. Coomassie staining is less sensitive but saturation of bands is sufficiant to quantification. Easy pre-labelling procedure combined with ist high sensitivity and excellent quantification properties make SERVA Lightning Red to the fluorescence stain of choice in SDS PAGE gel staining.
SERVA Lightning Red has a number of advantages:
- Direct detection
- No staining and washing steps after the run
- Very high sensitivity
The SERVAGel TG PRiME minigels are precast gels in cassettes for the vertical electrophoresis of proteins and nucleic acids. The gels are modified Laemmli gels - ...
The SERVA BlueShake is flexible applicable in various incubation and preparation techniques (e. g. staining/destaining procedures).Due to the large incubation platform ...
Gel staining apparatus
The new SERVA BlueStain automates the staining and washing steps when dyeing polyacrylamide gels. This universal device is used wherever ...
BlueSpin Cryo Microcentrifuge- refrigerated centrifuge with samll footprint!The BlueSpin Cryo is a refrigerated compact benchtop microcentrifuge for all kinds of ...
BlueSpin Mini Microcentrifuge- now you can spin!The BlueSpin Mini is a small benchtop microcentrifuge for all kinds of sample preparations and molecular biology ...
HPE BlueHorizon- optimized performance in cooled flatbed gel electrophoresis.
HPE BlueHorizon is a flatbed system for horizontal electrophoresis using precast gels, ...
HPE BlueTower System- enabeling high performance electrophoresis
The SERVA HPE BlueTower System is a multilevel flatbed electrophoresis device providing unmatched ...
BlueVertical PRiME- high quality mini gel tank systemThe new BlueVertical PRiME electrophoresis chamber is a perfect instrument for vertical mini gel electrophoresis ...
The SERVA Musketeer is an advanced gel documentation system with user-friendly features, easy operation and quick response. This imaging system consists of a scientific ...