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SERVA Lightning Red for 1D SDS PAGE

Logo SERVA Electrophoresis


SERVA Electrophoresis

Exhibitor details
Exhibitor details
Logo SERVA Lightning Red for 1D SDS PAGE

Product description

SERVA Lightning Red is a fluorescent dye for rapid labelling of proteins prior to SDS PAGE, making any staining and washing steps after electrophoresis unnecessary. In addition the dye is fully compatible with mass spectrometry and other downstream methods e. g. Western Blotting. SERVA Lightning Red is compatible with all additives typically used for sample solubilisation and protein extraction, including reductants like dithiothreitol (DTT) and dithioerythreitol (DTE).The shift of the electrophoretic mobility in SDS electrophoresis is very low; migration differences between labelled and non-labelled proteins could not be detected. This is due to the small mass addition of only 288 Da per bound molecule and the low hydrophobicity of the dye molecule. Traditionally silver staining of PAGE gels is preferred to other staining methods when highest sensitivity of detection is required. However silver stained bands exhibit very quickly saturation. Therefore quantification of proteins is not feasible. Coomassie staining is less sensitive but saturation of bands is sufficiant to quantification. Easy pre-labelling procedure combined with ist high sensitivity and excellent quantification properties make SERVA Lightning Red to the fluorescence stain of choice in SDS PAGE gel staining.
SERVA Lightning Red has a number of advantages:
- Direct detection
- No staining and washing steps after the run
- Very high sensitivity

Hall 20, Stand B27

(Main stand)

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