LABVOLUTION Digital Edition |
The complete V3 Western Workflow incorporates traditional blotting techniques with innovative tools to quickly check electrophoresis results and blot transfer quality prior to western blotting. This simplifies total protein quantitation analysis post-immunodetection and obtains results faster.
Separate proteins — Mini-PROTEAN TGX Stain-Free Precast Gels and Criterion TGX Stain-Free Precast Gels offer superior protein separation with fast run times and use standard Tris/glycine running buffers. TGX Stain-Free Precast Gels contain a unique technology formulated into the gel chemistry that enables rapid, high-quality separation of proteins in as little as 15 minutes.
Visualise proteins — protein separation can be visualised and confirmed using stain-free technology, which covalently binds proteins, after a 1-minute activation on a ChemiDoc MP or ChemiDoc Imager. This allows the immediate visualisation of proteins across the whole gel without staining. Stain-free technology is a sensitive, time-saving alternative to traditional Coomassie staining, offering a streamlined workflow.
Transfer proteins — the Trans-Blot Turbo System is a rapid protein transfer apparatus that can reduce transfer protocols to as little as 3 minutes while maintaining high-efficiency protein transfers across a wide range of molecular weights.
Verify protein transfer — the ChemiDoc MP or ChemiDoc Imagers, paired with stain-free technology, enable instant verification of protein transfer. After transfer, immunodetect your protein of interest using Precision Antibodies coupled with Clarity or Clarity Max ECL Substrates.
Validate and quantitate — the fifth step, available only with a ChemiDoc MP or ChemiDoc Imager and Image Lab Software, may be used to validate western blotting data via total protein normalisation. When normalising to total protein, stripping and reprobing is no longer necessary. Image Lab Software gives quantitative results instead of relative normalisation.
Accelerating Scientific Discovery.
Digital PCR is a breakthrough technology that provides ultrasensitive and absolute nucleic acid quantification. It is particularly useful for low-abundance targets, ...